Monday, September 30, 2019

Why Did Civil War Break Out in 1642

Lack of Money One of the reasons why the civil war broke out in England in 1642 was because of Charles' lack of money. To discover the source of this, we have to go back to the beginning of James' reign. James was the first King to reign over both England and Scotland, and when he came down from Scotland it is said that he was astonished at how rich England was, while James had needed to borrow money for his travelling expenses. When James died in 1625, Charles came to the throne, and he, like his father, had very little money.Once Charles became King, the County Faction1 wanted him to go to war with the Catholics in Spain, so Charles asked them for taxes to use on the war. They refused to pay enough, so the war was hopeless, and Parliament blamed the King for this. The reason Parliament granted so few taxes was that they wanted to make sure they were called again. Charles, a firm believer in the Divine Right of Kings, thought that he should not have to rule with Parliament, and the only thing that kept him calling it was money2. One good example of the way Parliament made sure they were called back in Charles' reign was tonnage and poundage.These were duties imposed on certain imports and exports. It was normal for these duties to be decided in the first Parliament of a monarch's reign, but in the case of Charles, they only decided on it for one year, so the King would be forced to call them again. Although Charles tried to ask for more money, Parliament refused, because they believed he spent it on his favourites. Because of this, Charles had to get himself more money. He began using the Church Courts, exploiting taxes such as ‘ship money'3, and selling monopolies and titles. He also opened a Court of Star Chamber, which he used to fine people heavily to raise money.Since the judges in the Star Chamber were officials of the Crown, and there was no jury, Charles could be sure of getting a favourable result. Parliament was furious with this, and immediate ly drew up the Petition of Right, which asked the King to stop illegal taxation. The King signed it, but only because Parliament threatened to impeach Buckingham, one of the King's favourites. The quarrels about money went on, and eventually Charles decided to dissolve Parliament. He reigned without them for 11 years. When the new prayer book was brought into Scotland, a group called the Covenanters attempted to invade England.Charles called a Parliament to try and get taxes to fight the Covenanters, but they refused4, so Charles dissolved them again. He was forced to pay the Scots ? 850 a day to stop them advancing, and eventually, in 1641, his money ran out, and he had to call Parliament – he was bankrupt and at their mercy, so money was definitely a key factor in the outbreak of the civil war. Religion Another major influence in the outbreak of the civil war was religion. The religious quarrels began right at the start of Charles' reign, when Charles married Henrietta Mari a, a French Catholic.Although Charles didn't choose to marry her – his father, James, set up the marriage – the public, especially the Puritans, didn't like having a Catholic as Queen. A few extremists even saw this as a sign that Charles was secretly Catholic! After the King dissolved Parliament, he made William Laud the Archbishop of Canterbury, in 1633. While Laud was Archbishop, he made many changes to the Church. Most of these changes involved beatifying the Church and bringing back robes for priests, statues and stained-glass windows. All these things reminded the English of Catholicism.In 1636, Archbishop Laud decided to introduce the English Prayer Book (which stated how services should be run) into Scotland. There was nationwide rioting, because no one wanted to follow the new Prayer Book. Scotland was a Presbyterian (Puritan) country, and they thought that the English Prayer Book was far too Catholic to use in Scotland. This eventually led to many Scots, call ed the Covenanters, marching down the country in an attempt to invade England. At this point, Charles had to call Parliament to ask for taxes to pay for the war, but was horrified to see that most of the MPs were on the Covenanters' side.Parliament agreed that the Prayer Book was too Catholic, so Charles dissolved them again, but after he ran out of money to pay the Scots (see the Money section above) he was forced to call Parliament again. Parliament first put Laud on trial, and found him guilty. Later they decided to execute Strafford on charges of organising an army in Ireland, where he governed. It turned out that this was a big mistake. As soon as Strafford was executed, the Irish Catholics rebelled against the Protestants, saying they were rebelling for the King.Although it was clear this was not true, Parliament did not trust the King when he asked them for an army, and so refused, believing he would use it to crush them instead. Foreign Affairs Another factor in the outbreak of civil war was foreign affairs. On the continent, the 30 Years War was going on, where Catholic rulers attempted to wipe out the Protestants in their countries. This fuelled people's fears that something similar might happen in England. Other foreign causes of the war were from Ireland and Scotland, and are detailed above. Charles' PersonalityAlthough it may seem unimportant, Charles' personality was a major factor in the events leading to civil war. To start with, Charles hadn't expected to be King at all – his elder brother, Henry, had been expected to take the job, but when he died suddenly in 1612, Charles became the heir to the throne. Charles also believed in the Divine Right of Kings. He thought, as his father had before him, that Kings were appointed by God, and could not be wrong. He disliked having to rule with Parliament, and thought that he should be able to do anything he liked. This caused much friction between him and the MPs. Parliament The Short Parliament When Parliament was summoned in April 1640, Charles had governed for eleven years without them5, and while this certainly went against the spirit of the English constitution, it was within the King's prerogative to do so. Charles was forced to call a parliament when the Scots rebelled. Putting an army into the field to deal with the Scots put a heavy drain on the royal finances, so Charles needed to levy a tax, which he could only do with the consent of Parliament. When Parliament met the gentry from the counties used the occasion to vent their frustration, with Harbottle Grimston and John Pym leading a catalogue of complaints.Three weeks later, Charles dissolved Parliament, blaming ‘the malicious cunning of some few seditious affected men'. This was known as the Short Parliament and it sat from 13 April, 1640, to 5 May, 1640. The war with the Scots did not fair well for Charles, but he eventually came to an agreement of ? 850 a day to keep the Scots at bay. The Long Parliamen t In order to pay this and get funds for a final settlement Charles had to summon another Parliament6. This time Charles could not afford to dismiss Parliament until he got what he wanted, and this gave Parliament an important card to play in what was to come.The Long Parliament, as it was known, sat from 3 November, 1640, for 13 years, until Oliver Cromwell suppressed it. It should be noted it was not formally dismissed until 1660, after the Restoration. Therefore, when Parliament met in November 1640, it was with a mood of constitutional reform. Of the 493 MPs elected: * 340 were anti-Court (the County Faction) * 64 were for the Court (supporters of the King) * 59 were of an unknown disposition * 17 were disabled from sitting (these were mainly of the ‘Court Camp') * The remaining 13 were probably of the Court Faction.Parliament pinned the blame for what went wrong on the King's advisors, rather than Charles himself, sending both Strafford and Laud to the Tower. Charles also tried to heal the rift by signing Strafford's death warrant, passing a bill that allowed for Parliament not to be dissolved without its own consent, a bill making ‘ship money' illegal and other bills that taken together demolished the framework of prerogative government. The Thrilling Climax All of these causes led to some key events in 1641 and 1642. It turned out that the execution of Strafford had been a mistake. Without Strafford to reign over Ireland, the Irish rebelled in 1641.This raised an insoluble problem; who would command the Army, King or Parliament? John Pym took the initiative by issuing the Militia Bill and, more importantly, the Grand Remonstrance. It listed all the things Charles had done wrong in his reign, suggested less power for bishops, and said that Parliament should have power over the Church and the appointment of Royal ministers. It was passed by 11 votes, which meant that, while most of the Commons had previously been against him, now almost half o f them supported him. However, it was after this that the King made a foolish move.On the advice of his Queen (who was used to French politics, where the King had much more power over the way the country was run), Charles decided to arrest the five ringleaders, including Pym. On 4 January, 1642, Charles attempted to get into the Commons to arrest the five MPs, and found that after he and his guards had battered the door down, the MPs had been warned and weren't there. This action turned most of Parliament against him once more, because it was held to be a breach of Parliamentary privilege7. On the next day, the escaped MPs paraded up and down London guarded by the Trained Bands, an army of part-time soldiers.

Sunday, September 29, 2019

People’s Republic of China Essay

Mainland China is rooted in more than 4,000 years of rich Chinese cultures and history. It’s known for their lives, travel, history, business and food, their people and customs. Traditional Kung Fu is as much of the culture of china according to statistics. The Chinese people have shared a common culture longer than any other groups on earth. China is one of the cradles of the human race. For thousands of years the culture of china has attract and moved many people from all over the world, being so unique and one-of-a-kind as well as elegant and inspiring. The Chinese culture, a culture that has evolved for thousands and thousands of years contains rare beauty and enchanted with history variously as an ancient civilization extending over a large area in East Asia. [pic] In order to effectively examine China we need to first understand what their culture is about. Culture is defined as â€Å"society’s shared and socially transmitted its ideas, values, and perceptions, which are used to make sense of experience and which generate behavior that is reflected in that behavior†. There are five major characteristics of culture, they are that; Culture is shared, â€Å"one shared element found in all cultures is an understanding in regard to gender roles, which are the roles a particular culture assigns to men and women†. The second characteristic is â€Å"Culture is learned, not biologically inherited. The process whereby culture is passed from one generation to the next. The third is â€Å"symbols. Much of human behavior is mediated by symbols—signs, sounds, emblems, and other things that represent meaningful concepts. † The forth is that â€Å"Culture is integrated. The foundation of culture includes three structural elements that work together to keep the culture strong: An infrastructure provides the basic necessities of life, a social structure determines how people interact with one another, and a superstructure, or worldview, provides a belief system that helps people identify themselves, their society, and the world around them. † Lastly, â€Å"Culture is dynamic. Chinese arts and crafts, including painting, calligraphy, operas and silk. During these four or five thousand years of development, china mainly live on the five common cereals and vegetables and added by a small supply of meat which is radiated to the advance of culinary skills and early civilization of the country. When one element within the system shifts, the entire system shifts to accommodate it. † East Asian cultures have many similarities in religious beliefs, family social networks, diet and education. Modern China differs greatly in government. They have maintained a communist state and resisted Westernization. This is not the case with the rest of the Far East. Western culture appears to be a driving force behind most of modern East Asian culture. [pic] China has a very diverse geographical and urban landscape. Within China’s borders lies the tallest peak in the world, Mount Everest, it is just one of many mountains that make up the Himalayan mountain range in the northern region of China. China also boasts the third longest river in the world with South China’s Yangtze River which is approximately 3,400 miles long. China has over 8,700 miles of coastline, and has many plains and valleys throughout its nearly 12,400 square miles of frontier land. The vast landscape has dwellings that vary anywhere from small villages to large cities and metropolitan areas. China also has one of the largest man-made structures in the world, the Great wall; it is so large that it is visible from outer space. â€Å"In terms of climate, China may be divided between the humid eastern region and the dry west. The humid east can be further subdivided between the warm and humid south and southeast and the temperate-to-cool, moderately humid north and northeast. Much of the humid eastern region of China exhibits a monsoonal pattern of temperature and precipitation. In a monsoon climate, the warm summer months are typically the months of maximum precipitation. † China’s population exceeds 1 billion, â€Å"more than 500 million people live in and around cities in China, according to the 1990 census. There are more urban dwellers in China than are found in either the United States or Russia. Some of the cities are quite large. Shanghai, for example, has more than 7 million people; Beijing has more than 6 million, and Tianjin more than 5 million. Many cities have more than 1 million people each. † (Compton’s Living Encyclopedia) China’s cities are expanding rapidly. The government is attempting to regulate urban growth in order to avoid such problems as congestion, overcrowding, slum development, and unemployment. China’s government is a People’s Republic; the main organs of the government are under Communist party control. The head of state in China is the â€Å"Premier†. The population of China is and has been a strain on the countries resources; because of this the government has interjected itself into the people’s family planning. â€Å"China’s family planning policy combines government guidance with the wishes of the masses. The basic requirements of family planning are late marriages and late child-bearing, so as to have fewer, but healthier, babies, especially one child per couple. But a flexible family planning policy is adopted for rural people and ethnic minorities; in rural areas, couples may have second baby in exceptional cases, but must wait several years after the birth of the first child. † Chinese people have the tradition of respecting the old and loving the young. Though many young couples do not live with their parents, they maintain close contact with them. Grown up children have the duty to support and help their parents. The Chinese people attach great importance to relations between family members and relatives, and cherish their parents, children, brothers and sisters, uncles, aunts and other relatives. Communication among the people of China can be challenging due to the numerous written and spoken languages of the country. â€Å"The Han people have their own spoken and written language. Chinese belongs to the Han-Tibetan language family. It is the most commonly used language in China, and one of the most commonly used languages in the world. All of China’s 55 minority people have their own languages, except the Hui and Manchu, who use Chinese; 23 of these have a written form. † Economically China has changed considerably since it became a communist state in 1949, since the founding of New China, especially in the 20 years after the start of reform and opening to the outside world in 1978. China has made great achievements in economic construction and social development. China instituted multiple financial plans that has allowed for outward economic growth and modernization. â€Å" There are three major religious in china; Confucianism, Taoism and Buddhism, although Confucianism is a school of philosophy than a religion, some turn to Taoism when they find themselves frustrated and many turn to Buddhism even if they never read the sutures beliefs. Chinese people do not have a strong religious inclination but despite this the three main faiths. China is proud of the many people, The Hui, Uygur, Kazak, Kirgiz, Tatar, Ozbek, Tajik, Dongxiang, Salar, and Bonan people adhere to Islam; the Tibetan Buddhism, and the Dai, Blang and Deang to Theravada Buddhism. † Buddhism was first introduced to China from India in the first century A. D. , becoming increasingly popular after the fourth century. Tibetan Buddhism, or Lamaism as it is sometimes called, is found primarily in Tibet and Inner Mongolia. War and the military have been a central role in the shaping of modern Chinese history; armed struggle has played an important part. â€Å"The majority of China’s imperial dynasties rose and fell due to success or defeat upon the battlefield. The 20th century saw the conflict between Nationalist and Communist forces that resulted in today’s divided China: the Peoples Republic of China (PRC) and Taiwan. It also witnessed China’s intervention in the Korean War and border clashes with its neighbors India, the Soviet Union, and Vietnam. † (Worthing) Many factors have led to the perceived military threat known as China. These factors have figured decisively in the rise and fall of imperial dynasties, the suppression of internal revolts, the emergence of revolutionary movements, and the conduct of China’s foreign relations, the development of political parties, the structure of governments, the rise of nationalism and the drive for modernization, all of which have driven the worlds perception of China emerging as a 21st-century military and economic power. China’s past has aided in shaping much of the countries core values, but economically a western view on commerce and technology has been adapted. China’s determination to do all outward dealing on their own terms has allowed them to be a major player in the world marketplace. China is self sufficient and goal oriented. They are a country diverse in belief systems yet this does not appear to affect the people’s sense of nationalism. China is a superpower that no country should overlook. The Chinese culture is solely based on the necessities of ancient Chinese culture. Which are: food, clothing, housing, transportation and, education. These elements are the foundations that make up their society, tradition and beliefs. Their beliefs, not only help shape their economic climate, it also influenced their military. China has the longest period of continuous development of military culture of any civilization in world history. China also had one of the most powerful and advanced military for almost 2000 years until the eighteenth century. One of the greatest influences of military thought was the book â€Å"The Art of War† by Sun Tzu’s. Of course a lot has changed since the 18th century. The China’s modern military share some of the same values of U. S. military. The 3 main beliefs, Confucianism, Taoism and Buddhism still play a major role in their decision making process. However, similar to the U. S. politics plays a major role overall. If we were to come in contact with any Chinese forces we must remember what is sacred to them. While most of us place value on religion from a â€Å"supernatural force†. The Chinese listen and respect their elders for wisdom and guidance. Here is a chart that shows you the difference between Chinese cultures vs. American culture: | |Chinese |Americans | |Conception Of the Self |Collectivist: Higher value placed on group|Individualist: Higher value placed on | | |cooperation and individual modesty. |self-reliance. Self-promotion is more | | | |accepted. High value placed on â€Å"freedom† | | | |from externally imposed constraints. | |Social Relationships |Formal, hierarchical. People most |Informal, egalitarian. People most | | |comfortable in the presence of a hierarchy|comfortable with their social equals; | | |in which they know their position and the |importance of social rankings minimized. | | |customs/rules for behavior in the | | | |situation. | | |Friendship |Small numbers of close, lifelong friends |Large collection of â€Å"friends† and | | |who feel deeply obligated to give each |acquaintances which changes over time and | | |other whatever help might seem required. |involves only limited mutual obligations. | |Obligation |Relationships with other people involve |People avoid interdependent relationships | | |reciprocal obligations. |and situations that might entail long-term | | | |obligations. | |Task vs. Relationship |Relationship-oriented: Maintaining a |Task-oriented. Relationships are less | |Orientation |harmonious relationship has priority over |important than getting the work done. | | |accomplishing tasks. | | |Harmony vs. |Avoid direct confrontation, open |Willing to confront directly, criticize, | |†Truth† |criticism, and controversial topics. |discuss controversial topics, and press | | |Concern maintaining harmony and with |personal opinions about what they consider | | |†face. † |†the truth. Little concern with â€Å"face. † | |Role of laws, rules, and |More faith in personal relationships than |Written rules presumably apply to everyone | |regulations |in written rules and procedures for |and are assumed to produce fair, reasonable| | |structuring interactions. |procedures and decisions. | |Time Consciousness |Relatively more attention to the past and |Less interested in the past; eye on | | |to the longer-term future. |near-term future. | |Ascribed vs. Achieved |Traditionally, a person’s status in the |People’s status is based mainly on their | |Status |society was based importantly on inherited|own achievements, including education | | |characteristics such as age, gender, and |obtained and level of success realized in | | |family. This is changing. |their line of work. | In order for America to be successful we must consider our differences and find a common ground. Knowing that there is a cultural gap can and will affect unit operations but we can and will be successful if we understand that we do not live in this world alone.

Saturday, September 28, 2019

Are Advertisements Aimed at Teenagers Effective? And, are They Ethical?

Questions to research: Are advertisements aimed at teenagers effective? And, are they ethical? My Response: Most advertisements aimed at teenagers are effective, but usually are not ethical. Most marketers have many ways of gathering information on teenagers spending habits and what is most important to teens. With this information they’re able to create advertisements that will appeal to most teens and create profit. Many people argue that some or most of these ads aren’t ethical because they will create a problem or insecurity and then give the solution to that problem in the form of their product.Teens will listen to other teens in ads. http://www. utalkmarketing. com/pages/article. aspx? articleid=14465&title=teens-will-listen-to-other-teens-in-ads Central Idea: The most effective ads show teens using and enjoying a product. The least effective were those that caused an emotional response. A teen’s acceptance of ads varies according to the media platform. The medium with the lowest teen ad acceptance was texting. The â€Å"Teen Advertising Study†, compiled with the help of the University of Massachusetts, showed that teens were more susceptible to online ads if they could relate to it and if they found it funny.Advertising to Teens http://suite101. com/a/advertising-to-teens-a14289 Central Idea: Marketers love teens because they easily spend money on â€Å"luxury† items such as clothing, electronics, and music. They mostly make their purchase decisions independently, have significant influence on family purchases, and companies know that once they have â€Å"branded† a child, they are likely to be customers for life. They reach kids by advertising in magazines, movies, TV shows, and on the internet. Companies get info about kids spending habits from internet â€Å"quizzes† and â€Å"surveys†.Marketers know how to capitalize on important teen issues and anxieties, like body image, peer acceptance, cooln ess, and need for power. Marketers often use a theme or attitude like sex or alcohol and drug use that seems to raise the â€Å"coolness factor† of their product. Advertising is so effective because it creates insecurities about things such as appearance. Successful ads convince the viewer that they have a problem and then offer the solution, which is the product they’re selling.This gives off the messages that teens aren’t good enough the way they are and many kids unwillingly buy into that message which is causing kids to grow up in the most materialistic society we’ve ever had. Most of the things that are advertised to teens do not promote healthy development.Central Idea: In one year, teens view more than 40,000 ads on TV alone while also being exposed to ads on the internet, in magazines, and in schools.Teens are often the main target for marketers because they are always willing to spend money on the newest â€Å"luxury† items. Teens strive t o have the newest electronics, clothing, and other overly advertised items. Marketers know how to target teen issues such as body image, peer pressure, and the need for acceptance. On average, teens are exposed to 3,000 ads per day. Teenagers, on average, spend $155 billion per year on advertised products, also influencing their parents to spend another $200 billion per year. Recent studies have shown tobacco companies use their ads to target teens as young as 13-years-old.Another major advertiser is the alcohol industry, using ads that feature young, attractive people drinking or posing with their beverages. Ads affect teen psychologically by exposing them to ads that suggest they are not what the entertainment business considers attractive. Ads can be harmful to teens by lowering their self-esteem. Children, Adolescents, and Advertising http://pediatrics. aappublications. org/content/118/6/2563. full Central Idea: Children and teens view 40,000 ads per year on TV alone even though the Children’s Television Act of 1990 limits advertising on children’s programming to 10.5 minutes/hour on weekends and 12 minutes/hour on weekdays.M-rated video games, which are not recommended for children younger than 17-years-old, are frequently advertised in movie theaters, video game magazines, and publications with high youth readership. Young people see 45% more beer ads and 27% more ads for hard liquor in teen magazines than adults to in their magazines. An increasing number of web sites try to get teens to make direct sales. More than 100 commercial websites promote alcohol products.Most advertisers use techniques that children and teens are more vulnerable to, like product placement in movies and TV shows, action figures, kid’s clubs, and celebrity endorsements. Sex is used in commercials to sell almost anything now. American ads constantly use thin female models, which contribute to the development of self-esteem issues in young girls. Thesis Statem ent With marketers aiming their advertisements more towards teens every day, researchers are looking into how effective and ethical these advertisements really are. OutlineThe Ethics and Effectiveness on Advertising toward Teens Thesis statement: With marketers aiming their advertisements more towards teens every day, researchers are looking into how effective and ethical these advertisements really are. I. Teens are being exposed to advertisements more each day. a. . Companies gather info about teens’ spending habits from internet â€Å"quizzes† and â€Å"surveys†. b. Children and teens view more than 40,000 ads per year on TV alone. c. More than 160 magazines are now targeted towards teens. II. Different factors will make an ad effective.a. Teen’s acceptance of ads depends on the media platform. i. Sponsorships of live events had the highest level of acceptance. ii. Text messaging had the lowest level of acceptance. b. Least effective ads were those tha t caused an emotional response.The advertising strategies marketers use have been shown to be unethicalM-rated video games are frequently advertised in movie theaters, video game magazines, and publications with high youth readershipYoung people see 45% more beer ads and 27% more ads for hard liquor in teen magazines than  adults to in their magazines.Advertising is so effective because it creates insecurities about things such as appearance.American ads constantly use thin female models, which contribute to the development of self-esteem issues in young girls.Gives off the message that teens aren’t good enough the way they are.Body Paragraphs I believe that the advertisements aimed at teens are effective, but unethical. Through a number of research tactics marketers are finding ways to keep up with the ever changing trends in teen culture.With the information they gather they’re able to produce advertisements that ultimately pressure their audience into buying their product. With marketers aiming their advertisements more towards teens every day, researchers are looking into how effective and ethical these advertisements really are. Technology based advertisements are effective among teens because of how often they are exposed to ads, the ads appeal to their lifestyles, and even sometimes pressure teens into buying their products. Advertisements have rapidly been integrated into the world around us.â€Å"Young people view more than 40 000 ads per year on television alone and increasingly are being exposed to advertising on the Internet, in magazines, and in schools. † Teens are heavily influenced by these ads because of how often they are exposed to them. Marketers are gathering information about teens’ spending habits through quizzes and surveys. ‘Teen Advertising Study’, which was compiled in conjunction with the University of Massachusetts, revealed that teens were more susceptible to online advertising if they co uld relate to it and if they found it funny. †With information like this they’re able to determine what type of ads would be effective on the teens of today. Different factors can make an ad effective, such as how they’re viewed and if it’s relatable to their audience. The most effective ads showed teens using and enjoying their product. For example, in this PlayStation 4 Interface commercial, you’re shown teens happy with their product and you’re given a glimpse of some features. This is considered an effective ad because it features the newest upgrade of their product, they’re being shown new features and games, and seeing other teens happy  with the product would give them a positive feeling about having it.This ad also shows both genders enjoying their product, suggesting they support both genders using the PS4. An example of an ineffective ad would be the Microsoft Surface commercial, in this commercial you can see people enjoyi ng their product but you’re not given a look at what their product can do. It’s a fun ad to watch, but it doesn’t provide enough information for a teen to considering buying their product. Lastly, many advertisements aimed at teens have been said to be unethical in order to be effective.Marketers will bring up a problem in their advertisement; this problem might be based on social status, then they will provide a solution in the form of their product. For example, in this Samsung Galaxy S4 commercial you see only younger people using their product while the older people are just learning about this product. This suggests that if you have their product you’d be cool and up to date with the world around you. This ad seems to guilt the target into thinking what they have isn’t good enough and their product would make them cooler. Conclusion In conclusion, I think advertisements aimed at teens are effective, but unethical.Teens are being exposed to adv ertisements more each day. Advertisements can be found all around them, in schools, online, through T. V. , etc. Marketers are constantly doing research to improve ads to be more appealing to their target audience, teens. Through the information they gather, they can create advertisements that will persuade their audience into buying their product. An effective ad would feature other teens enjoying their product. An effective, but unethical ad, would be an ad that brings up a problem, such as a teen’s social status, and then present a solution in the form of their product.

Friday, September 27, 2019

Centerofthequadrangleduewestof UphamHall ontheMiamiUniversityCampus Essay

Centerofthequadrangleduewestof UphamHall ontheMiamiUniversityCampus - Essay Example The sun rises in the morning and spreads its rays on all objects across the globe; the sculpture is no exception. The sky was clear blue and humidity was at its lowest. The temperature was just beginning to rise and clouds that had heavily hanged over my head ran away to create a path for the sun. With the help of the sunlight, every single image seems clear and the vicinity of the sculpture is quite detailed. One advantage with the day-light is that every facet of the sculpture can be vividly observed. The bracketing and columns stand very firm to support the compact oval roof-like capital (Cothren, 451). The central-plan building is crystal-clear and I could easily see and appreciate the outstanding work of the burin used by this legendary sculptor. In the chilly evening, the sculpture seemed to have had absorbed some rays from the sun. And now the time came to spit the rays, like a fire dragon. I could not stop dreaming as watched the sculpture reflect a few rays of the sunset. I stood from a close range and watched the beautiful colonnade majestically rise from the very bold and energetic dado (Brookes, 37). A first, I imagined a dwarf on a stand-still; any coward man would run away as he comes to face with the statue. However, the dimness of the evening denied me the sight of the beautiful cameo and chamfer of the capital. In as much as the sculpture was quite prominent in the evening, a few details of it could not be observed. The sculpture is located a stone-throw away from the main administration block of the institution. It borders the pavement that connects a number of buildings within the institution. Many trees have gathered around the earthwork to behold its unending beauty. Being by a path, it was easy to locate. From far, however, one could not easily spot it since it is surrounded by a building and some trees. The management of the institution may have strategically placed it here so as to

Thursday, September 26, 2019

Developing a Multi-Skilled, Customer-Focused People Management Essay

Developing a Multi-Skilled, Customer-Focused People Management Approach in the Middle East through Reducing Dependence on Foreign Workers - Essay Example The paper tells that not like the trends of development elsewhere, the transition of the economy of the Middle East or the Gulf Cooperation Council (GCC) has been largely driven by the trading in of expatriate foreign employees. This pattern will not cease for the near future, although at a slower rate, but the involvement of foreign employees in the course of transforming political and economic institutions will demand, inevitably, multi-skilled and customer-oriented people management techniques. Large corporations and government bodies in the Middle East, as well as international agencies, are vaguely informed of such regional trends, but the implications of such developments for human resource management (HRM) is not widely recognized or understood. By conducting an appropriate evaluation of the factors and patterns influencing development, this paper tries to contribute to the knowledge required by large corporations in the Middle East to create a more systematic, multi-skilled, and customer-oriented workforce management approach. Changing economic progress and lessening reliance on foreign employees are the key tasks confronting the Middle East. Not like elsewhere, the Middle East has too little literature on HRM. A detailed review of available literature reveals the lack of any methodical assessment that might give a broad image of the HRM mechanisms in the Middle East. In fact, there is hardly any trustworthy country-specific research that has surfaced with the economic progress of a specific Middle Eastern country. For instance, management and HRM approaches in Saudi Arabia, people management in Turkey, organizational and HRD success in Israel, international business and management problems in Jordan, and workforce management in the GCC (Peterson 1993). Moreover, the available literature emphasizes several studies associated with development and training in the Middle East. Ali (1996 as cited in Budhwar & Mellahi 2006), for instance, focuses on the inad equacy of attempts exerted by professionals to make sense of Arab management approaches and their effect on the success of organizational development programs and cross-cultural cooperation in the region. Likewise, a number of researchers talk about the success of management training and its effect on managerial competency in various Middle Eastern societies (Scullion & Collings 2010). As stated by Briscoe and Schuler (2004), several academics have stressed the need for and processes of multi-focused, customer-oriented workforce management approaches in the Arab world. A large portion of related literature on the Middle East is about the effect of Arab values and culture on management dynamics (Budhwar & Debrah 2001). Likewise, Kabasakal and Bodur (2002 as cited in Budhwar & Mellahi 2006), based on socio-cultural comparisons, classified an Arabic group, composed of Qatar, Kuwait, Turkey, Morocco, and Egypt. Countries in this group are emphasized to be very masculine, structural/hier archical, group-driven, and weak on future direction. In contrast, Ali (1995 as cited in Budhwar & Mellahi 2006) argues that multi-focused, customer-oriented organizations and management approaches in the Middle East can merely be built by taking proper account of the Arab context. He further argues that the foreign aspect is partly not favorable to the creation of multi-focused, customer-oriented management approaches in the oil rich Gulf States. Researchers have also explored the subject of management flow from the Western countries to the Arab world. Yavas (1998 as cited in Aswathappa & Dash 2007), for instance, studied the subjective value given to a cluster of management competencies by Saudi managers who had gained their business diploma in the United States. Yavas (1998) emphasizes several

Judaism and Shabbat Essay Example | Topics and Well Written Essays - 1500 words

Judaism and Shabbat - Essay Example In additional, they often believe that these laws and commandments were given to Moses both in written and in Oral Torah forms. However, a religious community, the Karaites movement, has historically challenged the notion that Moseswas given these laws and commandments in two forms (Herring 15). They have ever held that the laws and commandment given to Moses were only in a single form, that is, in written form but not in Oral Torah. The Karaites movement flourished during the medieval period and still has several followers in the contemporary world who have maintained that Moses was only given written Torah laws and commandments (Bank 22). Judaism has enjoyed historical continuity of over three thousand years; basically, three thousand eight hundred years since its foundation by Abraham. This makes it among the oldest monotheistic religions of the world that has survived until into the contemporary world (Miller 24). The Israelites or the Hebrew was already being referred to as Jews in the early books of Tanakh (Herring 24). The late books of Tanakh including the Book of Ester the name Jews were placed by the title the Children of Israel. The texts of Judaism, its tradition, and values had a strong influence on the subsequent Abrahamic religions that included Christianity, Baha’i Faith, and Islamic. ... By the year 2010, the entire world Jewish community was approximated to be roughly 0.2 percent or 13.4 million of the entire world’s population (Grishaver 71). Israel harbors around forty-two percent of the entire Jewish population. The same number is found in both United States and Canada with the majority of the remaining population found in Europe. Because of time and different understanding of concepts with a community, Judaism did split into movements. These movements rose due to different ideologies that might have cropped among the leaders of Judaism. The major Judaism movements of the world include Reform Judaism, Conservative Judaism, and the Orthodox Judaism. The latter movement includes sub-movements including the Modern Orthodox and Hareidi Judaism (Prager 77). There were believers who stark to the ideals of the conventional Judaism; thus, retaining the larger percentage of its teachings, beliefs, and laws. This group remained to the part of Judaism that was called Conservative movement believers who believed that the traditional and laws of Judaism should have only faced a slight changes to suit the time. The other movement that split out of the traditional Judaism was the Reform or Liberal, or the Progressive Movements. These sections of believers have the feeling that it is upon an individual believer to make a choice of the traditions and laws to follow (Herring 51). Therefore, the main course of the division may be pegged on the approach and concern to changes that should be enforced on the Jewish laws. However, Orthodox Judaism until to date maintains that the Jewish laws and Torah laws have defined origin, and are eternal and should never be changed; thus,

Wednesday, September 25, 2019

Business Operations Essay Example | Topics and Well Written Essays - 1000 words

Business Operations - Essay Example At the same time, it will concentrate on giving unique skincare diagnosis, facials, manicures, pedicures and other services, which are customized as compared to it competitors. Market segmentation is considered to the process in which customers are classified in accordance to their needs and objectives. It offers several advantages to an organization(Worthington, Britton & Rees, 2004). Firstly, it allows the company to concentrate on the main market. At the same time, it allows them to offer unique products and services. It assists the organization to emphasize and concentrate on its skills. Dove Spa will concentrate on targeting females of Streetly, between the ages of twenty and sixty. This segment has been selected because women between these ages are conscious about their looks and mostly take care of themselves. They used wide ranging beauty products. Dove Spa will give them skincare diagnosis, facials, manicures, pedicures and other services, which are customized. The competition is fierce in this industry and therefore, it is important that Dove Spa stands out unique as compared to its competitors. Marketing mix is considered to an important concept and it is the fundamental part of the marketing strategy. It concentrates on quality of services and products, which would be offered by the company. It concentrates on four essential components, which include price, product, position and promotion (Worthington, Britton & Rees, 2004). It concentrates on devising a marketing strategy, which would target every segment. The same strategy would be used for Dove Spa in order to advertise and promote it in effective way. It would assist the company to accomplish its aims and objectives. The main challenge for Dove Spa would be to develop an appropriate and strong business strategy in order to saturate itself in the market. Research suggests that the internal environment of the

Tuesday, September 24, 2019

Designing Effective Research Paper Example | Topics and Well Written Essays - 1500 words - 1

Designing Effective - Research Paper Example Based on this importance, the paper emphasizes the need for further research into opportunities presented by effective designing of contracts. This paper dissects how framing and expectations of contracts influence the contract’s performance. Various researches have attempted to look into the ubiquitous nature of contracts. Some have mentioned that contracts are merely necessary safeguards which secure parties involved in a contract against potential undesirable behaviors by the involved parties. This facilitates exchanges. Many scholars note that mitigation of the threats arising from contracts is dependent on contractual framing and expectations. Recent evidence from researches suggests contract framing play an important role in determining how contractual application is implemented. For instance, contracts which are framed with a loss in mind in case of failure to meet threshold amounts to the involved parties making greater effort. Similarly, contracts framed with a gain in mind, the parties will also be motivated to work even extra hard. The expectation of something is seen to motivate an action in implementation of the contract. Many contract theorists have begun systematic unpacking of behavioral implications associated with contract frame and expectations from the same (Weber and Mayer 56). A key emerging issue in design of effective contracts is the psychological impacts of framing and expectations of contracts. Despite widespread knowledge of the role of contracts in ensuring mitigation of hazards through development of safeguards, there exists little knowledge with regard to psychological impacts of the contracts. Contract framing offers a potential mechanism to psychologically affect the exchange or continuous partnerships.as revealed in the study, contracts aim at protecting the parties involved in a relationship against any form of opportunistic behavior thus boosting the confidence of the parties involved so that they can proceed with the

Monday, September 23, 2019

Business Description ( Adult Day Care ) Essay Example | Topics and Well Written Essays - 750 words

Business Description ( Adult Day Care ) - Essay Example Under medical services, the company aids, to provide medication reminders and helps with blood glucose test. The company projects to increase its operation by establishing subsidiary companies and small businesses in Los Angeles and other parts in the United State based on demand. In attempt to increase returns earned, the management shall increase the operations by diversifying its services. The company will also provide health-centered day care services. It will offer advanced medical care to seniors and adults with disability who are about to be placed to a nursing home. The parent company in Los Angeles will provide both services to seniors and adults with disabilities. Services to be offered by subsidiary companies will depend on exogenous factors such as market, competition, resources available among other factors. The company will have a centralized business structure with both leadership and top management heading the company. The organization structure of the company will have three important functioning areas: governance, administration and programs. The structure to be formulated will aid to enhance communication within the organization. In this perspective, the chain of command will flow from leadership, management downward to each day care center. In fact, the structure will be hierarchical and each system will be interconnected by chain of command. Presidents and fifteen member board of director shall head the subsidiary companies. The company will utilize both horizontal and lateral organization’s structure (Oklahoma  72). The main objective of the company is to offer high quality day care services that meet global requirements and customer’s satisfaction. Its major goal is to acquire global competitive advantage and increase revenues generated. The company will provide day care services through well equipped

Sunday, September 22, 2019

Do Woman Make Better Leaders Essay Example for Free

Do Woman Make Better Leaders Essay However, when women leaders are putting glorious greatly, they need to sacrifice their parts of life to earn such a success. Mostly, they need to work overnight and hard like their male fellows, they are single or divorced, and they may need to take care of both of their career and children. Actually, female leaders are facing some practical problems in their emotion, career and family. Many females are suffering in balancing these factors. Do women make better leader than men? Or specifically, are women playing a better role in managers? What are issues women leaders facing? In order to solve these problems and leave no doubt, I write this essay to analyze this topic. 2. 1 Leadership According to the professional definition, â€Å"leadership is the process of influencing an organized group toward accomplishing its goals. †(Roach Behling, 1984) There are certain basic qualities or characteristics that most people associate with leadership, such as self-confidence, persistent, integrity, ambitious and determination. People would agree that who own these qualities can be labeled as â€Å"leaders†. However, it is usually hard to recognize a proper leader with a standard measurement. Human traits are quantized immeasurable. In daily works, a good leader is normally judged by people he or she leads. Unfortunately, these opinions are always subjective and biased. Work performances may consider being comparable. However, in reality, only similar works can provide comparable performance results which limit the number of comparable cases. Insufficient parameters cannot provide a reliable analysis. Due to these difficulties, this essay will compare female and male leaders in other factors which are not specialized and quantized for leaders but also providing references for good leaders. These factors include effectiveness, intelligence and attitude which compare leaders in the aspects of performances; psychical and psychological; values and reliability, respectively. Before comparison and analyzing, it is more reasonable to realize the current situation about gender and leadership which will also give readers a general idea. 2. 2 Men are still the major force in leaders Actually, majority of world still consider that domination of power is in man’s hand, though women leaders are increasing rapidly recent years. As statistics show, woman account for 51 percent of population and 46. 5 percent of labor force while their numbers in manage level are negligible. In fact, in more powerful positions, women only account for 7. 3 percent of Fortune 500 corporate line officers; only 4. 1 percent of top earners are women. (Catalyst, 2001) The true situation is like what Oakley ,J. G. said,† The overall percentage of women on corporate boards surpassed the 10% milestone for the first time in 1996, however, 105 out of the 500 companies surveyed still had no women on their boards. †(Oakley, 2000; Dobrsynski, 1996) In making such a males’ world, social and environment factors play critical roles. Traditionally, women are told to be less competitive and ambitious when they are still girls, when they start their careers, mostly, environment and themselves keep suggesting them to be more unleader-like and docile. This attitude is formed in a long period and by surrounding environment, and it actually becomes part of female’s character. Attitude toward leadership is stronger predictor of leader emergence than masculinity. (Kolb, 1999) This theory is even more reasonable when biology theory is abandoned. Generally, majority of leaders is still male , the roles that women are told to play and attitudes they are suggested to own is signaling to be â€Å"second class†. 2. 3 Woman leaders show great potential It is undoubtedly men are traditionally leaders in the society; nevertheless, women are catching up in an amazing speed. Especially in recent years, woman leaders are showing their great power. In a long period before, people believe that women lack of managerial skills and they define it as â€Å"female deficiencies† as a reason why few women have made it to the top. Steven, Lynda and Joanne, 2003) However, many professors have proved the irrationality of this stereotypical concept, Davidson and Burke (DavidsonBurke,1994) have contended that almost all the evidence shows little or few difference in the traits and abilities of managerial skills among women and men. Furthermore, Ferrario(Ferrario,1994), Carless(Carless,1998) and Komives (Komives,1991) have studied and p roved that gender does not determine who should get the leadership. According to above statements, it is easy to realize that gender is not physical or psychical obstacle for female leaders. Moreover, Kent and Moss suggested the possession of feminine characteristics does not decrease an individual’s chances of emerging as a leader as long as the individual also possesses masculine characteristics. If women are more likely to be androgynous, they may have better chance of rising to leadership status. (Kent and Moss, 1994) Kent and Moss’ words can be explained related to women’s own characteristic. Normally, women are more approachable and modest. The advantage is obvious above; male leaders cannot combine female’s good personalities which will be considered to be sissy while females will treat androgynous as a merit which show their potential. So, in some kinds of careers which require managers to be more mild and communicative, such as teachers and nurses, woman leaders are more preferred. To sum up, women’s characteristic advantage is seen as particularly important in light of the trends toward flatter organizations, team-based management and increased globalization. Oakley,2000; Adler,1993;Rosener,1995) Undoubtedly, woman leaders have predominance in some specific works than man leaders 2. 4 Gender and the leadership 2. 4. 1 Intelligence comparison Intelligence is defined by Cronbach as a person’s all-around effectiveness in activities directed by thinking. (Cronbach, 1984) Intelligence can divide in two parts, academic intelligence and emotional intell igence. In the aspect of academic intelligence, it is well accepted that there is no difference in academic intelligence between men and women. Furthermore, people with high academic intelligence don’t mean they are good leaders. Obviously, traditional view about intelligence is quite narrow. Current research has denied the theory of IQ as the only measure of intelligence. In 1920 Thorndike hypothesized that true intelligence was composed of not only an academic component, but also emotional and social components. (Barbara Shilpa, 2003) Bar-on argued that emotional and social intelligences were better predictors of success in life. (Bar-on, 1997) According to Barbara Shilpa’s statistic analysis, the mean transformational leadership score of females and males are roughly the same (65. 21 and 63. 31 respectively). The mean emotional intelligence score for females was higher than the mean emotional intelligence score for females (109. 58 and 98. 31 respectively). (Barbara Shilpa, 2003) Although the results of the current study suggest that females have scored higher than males in emotional intelligence, it is possible that women as compared to men scored higher on empathy or social skills and low on motivation or self-regulation. However , male and female’s leadership score is very close which can prove that different genders have differences in EQ, but no capability difference in leadership. To sum up, there is no specific gender difference in academic intelligence. Females have a higher score in emotional intelligence and males have an equally emotional leadership score with females. 2. 4. 2 Attitude comparison Attitude means a general and enduring positive or negative feeling about some person, object or issue. (Kalliath, 2010) Normally, we divide attitude in two parts, one is the affective component, and the other one is the behavioral component. Actually, leadership is affected by attitudes in the sense that a leader’s attitude about their subordinates is dictated by theories X and Y. Theory X in the simplicity means leaders view their subordinates as pessimistic. They believe that the best way to treat subordinates is by manipulating for they think subordinates are lazy and unmotivated. Theory Y thinks oppositely, leaders of theory Y feel that most people are motivated and afraid of punishment. (Kalliath, 2010) Normally, leaders with a positive self-concept and a Theory X philosophy tend to be bossy, pushy and impatient; they also generally take on a dictatorial leadership. Leaders with a negative self-concept and a Theory Y philosophy may seem afraid to make decisions and self-blaming. Hughes, Ginnett Curphy, 1993) Related to above apart, women’s attitude is an obstacle for their leadership. This disadvantage is caused by environmental and cultural factors. All stereotyping views towards woman will affect them when they are becoming leaders. However, according to theory X and Y, they generally talk about two types of leaders. It can be seen that gender is not the major factor in attitude. To sum up, woman leaders are normally have more problems in their attitude and valuation than their man opponents. 2. 4. 4 Effectiveness comparison Effectiveness is understood in terms of leaders’ facilitation of a group or rganization’s ability to meet its goals. (Hunt, 1991; Mumford, 1986) It is stated that there is no difference in effectiveness, although some situations favor women and others favor men. (Powell, 1993)However, some people point out that female leaders may be less effective when they are doing stereotypically masculine jobs. It is easy to understand that women may violate conventions concerning appropriate female behavior. Female leaders will be subjected prejudiced reactions, which included biased performance evaluations and negative preconceptions about future performance. Consistent with research on self-fulfilling prophecies (Geis, 1993), negative opinion towards women’s leadership can diminish their performance. Even though, some professors argue that role conflict for female leaders might also be minimized by adopting a relatively feminine leadership style that would meet people’s traditional expectations about female behavior. (Eaglly, Makhijiani, Klonsky, 1992) They assert that it is unfair to value the effectiveness in leadership roles that are rarely occupied by women and in which most of their evaluators are male. (Eaglly, Makhijiani, Klonsky, 1995) In order to analyze this issue, two perspectives are introduced: alternative perspective and contingency theory based perspective. As a matter of fact, alternative perspective also have two parts, the first one is a structure view suggests that men and women are equally effective in leadership. (House, 1991Hunt, 1991) Another alternative view takes into account the different selection of men and woman for leadership. Besides gender equality opinion, it also points out that women may be superior performers, especially in the longer run as they may erode negative thinking about their work. Craig Jacobs, 1985) The second perspective is of contingency theories of leadership, suggests that leaders’ effectiveness depends on their style of leading. (Bass, 1990) It focuses more in the aspect of leadership style. It insists that effectiveness of different gender is related to corresponding factors in leadership methods, not gender. In a word, although male leaders are considered more e ffective in traditional masculine jobs, female leaders are more effective than males in less masculine terms. Effectiveness is a reliable proof of the woman leadership is not worse than man leadership. Although some people still believe woman leaders are using some inefficient management methods when they are most in masculine works. It can be expected that females will fare at least as well as men in an increasing range of leadership and managerial roles. 3. Conclusion From the days of old to the modern world, people who have the opinion that men are born to lead and women should serve as followers are out of date. No one can deny the gender equality in the current society. Leaders are no longer the exclusive privilege for men, but also for excellent females. We start to realize the importance of analyzing this topic and have taken a close look at some of the most basic qualities needed in a successful leader, such as attitude, intelligence and effectiveness. While some qualities seem to be more present in males than females, such as attitude, there are still many females owning the necessary characteristics to be better leaders, such as effectiveness in feminization jobs. This essay collects studies that analyzed leadership under rigorous researches and we have also taken a close look at some of the more recent studies on this topic.

Saturday, September 21, 2019

BoD Lipid Peroxidation Report

BoD Lipid Peroxidation Report A Study of lipid peroxidation The degradative process of lipid peroxidation in the liver and the potential of antioxidants to prevent cell damage Lipid peroxidation of rat homogenate using the Fenton reaction to generate free radicals (-OH and -O2) to initiate the self-propagating peroxidation of cell membrane fatty acids. Two separate antioxidants were used (aTocopherol and Quercetin) to study the potential of antioxidants in the prevention of cell damaged. Data of two separate groups (A+B) was provided along with data enabling the construction of a calibration curve to measure local MDA concentrations as an indication of peroxidation damage. The Fenton reaction produced the highest concentration of MDA in both data sets which is expected, allowing for a comparison of free radical damage in the presence of antioxidants. In the presence of aTocopherol, there was an MDA (nM/ml) concentration reduction from 45nM/ml to 24nM/ml evidencing a peroxidation inhibition via the binding of free radicals to the antioxidant though some damage was still caused as MDA concentration was higher than the control (7nM/ml). Quercetin showed a com plete reduction in local MDA concentration from 68nM/ml to 7nM/ml, which is equal to that of the control; evidencing a complete lipid peroxidation via the binding of all free radicals produced and thus prevents cell damage. Lipid peroxidation is the multistep process of oxidative degeneration of lipids. The process involved polyunsaturated fatty acids and the free radicals -OH (hydroxide) and -O2 (superoxide), which are unstable forms of oxygen to the incomplete valence ring on their outer shell resulting in an unpaired electron (free electrons). Due to the naturally unstable state of a single unpaired electron, free electrons are highly reactive (free radicals) requiring an electron to become stable; making the unpaired hydrogen atoms on the fatty acid tails suitable for binding (Mylonas C, 1999). The three step process (initiation, propagation and termination) of lipid degenerative produces highly reactive electrophilic aldehydes, which react with CH2 group forming CH (carbon centred) radicals. CH radical then reacts with O2 radicals producing peroxyl radicals (Yngo J. Garciaa, 2005). This propagation reaction then reacts with adjacent CH2 groups resulting in the formation of lipid hydroperoxide. Lipids are essential components of cell membranes (i.e. phospholipids and glycolipids) and can be used in the identification of damage as a result of the pathogenesis of disease via reactive oxygen species (ROS) concentration. ROS-dependent tissue damage can be identified by increased local MDA (malonedialdehyde) and 4-HNE (4-hydroxynonenal) (Kwiecien S, 2014). MDA is the product of lipid peroxides metabolisation, and can be indicative of oxidative stress related disease i.e. atherosclerosis, and induced gastric injury (due to gastric mucosa damage). Due to free radicals are reactive its uncommon that they a found in that state as they tend to bond and react very quickly in order to fill their valence shell and become stable. The Fenton Reaction (Fe2+ and H2O2) issued to generate free radicals (particularly -OH) and initiates lipid peroxidation within the liver. During the breakdown of lipids, malonedialdehyde (the final product of lipid breakdown) reacts with thiobarbituric acid resulting in a testable pink adduct. The Fenton reaction is as follows: Fe+2 + H2O2 > OH (hydroxyl ion) (Fenton Reaction) OH + lipid > malonedialdehyde Malonedialdehyde + thiobarbituric acid > thiobarbituric acid reactive substance (pink) Set up a series of test tubes a labelled and the volumes laid out in Table 1 were pipetted into the corresponding tubes. Remember to add the rat homogenate last due to this starting the reaction. The tubes were then incubated for 30 minutes at 37 degrees Celsius. At this point, the standard curve of MDA was set up as seen in Table 2 and tested at a wavelength of 532nm. After which thiobarbituric acid was added to the original test tube set and incubated for a further 15 minutes in after the adduct fluid was removed and tested at 532nm. Test Tube Test Buffer Tris HCL (00.2M) pH 7.2 FeCl2 H2O2 Catalase Quercetin OR aTocopherol Homogenate Total/ml 1 Control 1.6ml 0.9ml 2.5 2 Fe2+ 1.1ml 0.5ml 0.9ml 2.5 3 Fe2+/H2o2 0.6ml 0.5ml 0.5ml 0.9ml 2.5 4 Catalase/Fe2+/H2o2 0.5ml 0.5ml 0.5ml 0.1ml 0.9ml 2.5 5 aTocopherol or Quercetin /Fe2+/H2o2 0.5ml 0.5ml 0.5ml 0.1 0.9ml 2.5 Table 1: test tube volumes for each of the five test tubes in the lipid peroxidation assay, empty spaces indicated that the solution isnt added to that tube. Each was incubated for 30 minutes together under the same conditions. Test Tube Final MDA concentration (mM) Dilutions Volume of MDA stock (ml) Buffer (ml) Total Volume (ml) 1 0.1 Dilute 1mM MDA 1:10 0.3 2.7 3 2 0.05 Dilute 0.1mM MDA 1:2 (tube 1 extract) 1.0 1.0 3 3 0.01 Dilute 0.05 mM MDA 1:5 (tube 2 extract) 0.4 1.6 3 Table 2: The dilutions volumes of MDA and the final concentration required, these volumes were used to construct a calibration curve for comparison of the test samples in table 1. NOTE: all data using in the results was provided, this was due to an issue in the lab were where independent data was unintentionally taken by another individual and thus leaving no results for comparison against overall class data. MDA Concentration (nMoles/ml) Optical Density (OD) at 532nM 0 0 12.5 0.07 25 0.145 50 0.26 100 0.55 Table 3: MDA concentration (nMoles/ml), these values were used to construct the calibration curve Figure 1. MDA concentrations were provided due to an issue with both groups overall dilution series. The data from figure 1 was plotted using table 3. The R2 value (0.9986) indicates a strong linear value between the MDA concentrations (nM/ml) and the optical density. Figure 1: A calibration curve using the data from Table 3. The data set shows a strong linear relationship between optical density and known MDA concentration indicating good lab practice. Tube Mean -/+ Stdev SEM 1 Control 0.068 0.077 0.063 0.006 0.073 0.045 0.074 0.058 -/+ 0.025 0.010 2 Fe2+ 0.082 0.081 0.057 0.03 0.003 0.050 0.075 0.054 -/+ 0.029 0.011 3 Fe2+/H2o2 0.174 0.247 0.093 0.577 0.058 0.319 0.251 0.246 -/+ 0.173 0.065 4 Catalase/Fe2+/H2o2 0.355 0.169 0.246 0.063 0.056 0.143 0.134 0.167 -/+ 0.105 0.040 5 aTocopherol/Fe2+/H2o2 0.074 0.173 0.074 0.127 0.259 0.092 0.110 0.130 -/+ 0.666 0.025 Table 4: class data group A using aTocopherol, the values were done in repeat to gain a mean value and allows for Stdev calculation and thus SEM calculation, allowing for later comparison. The data set in Table 4 was provided and used the antioxidant aTocopherol. Seven repeats of each test were conducted to allow for a mean to be gained and thus a Stdev and then a standard error mean. The error mean allows for comparisons between different data sets as it indicates how accurate the experiment was rather than how varied (Stdev). The data was plotted in figure 2 and 3 with the variation of containing either the Stdev (figure 2) or the SEM (figure 3). Figure 2 allows for variation comparison while figure 3 allows for accuracy comparison between the two data sets (group A and Group B). Figure 2: the mean OD values of aTocopherol, the error bars show the variation within the data set. Test tube 2 was the most optically dense of the data set while test tube 2 was the least, though the error bar would suggest some variation in this value considering test tube 1 (control) was more optically dense. Figure 2 shows the optical density of aTocopherol. Test tube 1 contained only buffer and showed little variation between repeats resulting in a small Stdev, while test tube 4 has a large Stdev value and thus would need repeating in order to gain an accurate representation of the data. Test tube 3 was the most optically dense with a value 0.246 (at 532nm), while the OD went down between test tubes 4-5 (0.167 and 0.130). This is visually shown in in figure 3, where the data was plotted in a bar graph and SEM was used to show the accuracy of the experiment. The deviation of the error bars shows high accuracy in some results i.e. test tube 1-2-3. However, the deviation in test tubes 4-5 was high compared to other samples. Figure 3: the graph shows the class data of group A. The mean OD values of aTocopherol were plotted including the SEM to show how accurate the experiment was between data sets. Test tube 3 showed to be the most optically dense of the set while test tube 2 showed to be the least.   Ã‚   Tube Mean -/+ Stdev MDA concentration (nM/ml) 1 Control 0.058 -/+ 0.025 7 2 Fe2+ 0.054 -/+ 0.029 7 3 Fe2+/H2o2 0.246 -/+ 0.173 45 4 Catalase/Fe2+/H2o2 0.167 -/+ 0.105 28 5 aTocopherol/Fe2+/H2o2 0.130 -/+ 0.666 24 Table 5: a table showing the MDA concentrations of Group A class data set of each test tube using the calibration curve in Figure 1. Table 5 shows the MDA concentration of group A using aTocopherol, the control had the sample concentration of MDA as the Fenton reagent (7nm/ml); while test tube three which contained the Fenton reagent and H2O2 resulted in the highest MDA concentration of (45nM/ml). Adding the antioxidant resulted in a reduced MDA concentration of 24nM/ml. The visualisation of Table 5 data is seen in Figure 4 where MDA concentration is plotted against each test tube value (gained from the calibration curve) Figure 4: The graph shows the MDA concentration (nM/ml) of the groups A class data set, as only one set of samples was done no comparison can be made between the same antioxidant via Stdev. Test tube 3 showed to contain the highest concentration of MDA (45nM) while test tube 2 also showed to contain the lowest concentration of MDA (7nM). Tube Mean -/+ Stdev SEM 1 Control 0.041 0.06 0.08 0.057 0.057 0.02 0.297 0.087 -/+ 0.094 0.036 2 Fe2+ 0.037 0.039 0.06 0.06 0.053 0.074 0.047 0.053 -/+ 0.013 0.005 3 Fe2+/H2o2 0.28 0.704 0.242 0.365 0.247 0.385 0.528 0.393 -/+ 0.170 0.064 4 Catalase/Fe2+/H2o2 0.14 0.497 0.087 0.305 0.351 0.099 0.357 0.263 -/+ 0.156 0.059 5 Quercetin/Fe2+/H2o2 0.046 0.035 0.035 0.073 0.073 0.031 0.102 0.056 -/+ 0.027 0.010 Table 6: The table shows the class data set of group B using Quercetin as an antioxidant, multiple repeats were undertaken to allow for an average to be gained and Stdev and SEM to be calculated. The control only contained buffer solution. Figure 5: The graph shows the mean OD of the group B class data set, using quercetin as an antioxidant. Stdev values were used as error bars to visualise the variation between the dataset. Test tube 3 showed to be the most optically dense while test tube 2 showed to be the least though showed high Stdev and thus a lot of variation between the individual repeats. Figure 6: The graph shows the mean OD of the group B class data set, using quercetin as an antioxidant. SEM values were used as error bars to visualise the variation between the dataset. Test tube 3 showed to be the most optically dense while test tube 2 showed to be the least though showed high SEM and thus low accuracy between the individual repeats. Tube Mean -/+ Stdev MDA concentration (nM/ml) 1 Control 0.087 -/+ 0.094 15 2 Fe2+ 0.053 -/+ 0.013 7 3 Fe2+/H2o2 0.393 -/+ 0.170 68 4 Catalase/Fe2+/H2o2 0.263 -/+ 0.156 46 5 Quercetin/Fe2+/H2o2 0.056 -/+ 0.027 7 Table 7: a table showing the MDA concentrations (nM/ml) of Group b class data set of each test tube using the calibration curve in Figure 1. Table 7 shows the MDA concentration of group B using quercetin, the control had the sample concentration of MDA as the Fenton reagent (15nm/ml); while test tube three which contained the Fenton reagent and H2O2 resulted in the highest MDA concentration of (68nM/ml). Adding the antioxidant resulted in a reduced MDA concentration of 7nM/ml. The visualisation of Table 7 data is seen in Figure 7 where MDA concentration is plotted against each test tube value (gained from the calibration curve) Figure 7: The graph shows the MDA concentration (nM/ml) of the groups B class data set, as only one set of samples was done no comparison can be made between the same antioxidant via Stdev. Test tube 3 showed to contain the highest concentration of MDA (68nM) while test tube 2+5 also showed to contain the lowest concentration of MDA (7nM). NOTE: Due to individual data being lost only a comparison between the two data class data set can be made The enzymatic destruction (via catalase, superoxide dismutase) of membrane lipids is a crucial step in the pathogenesis of multiple disease states within adult (Mylonas C, 1999), the reactive oxygen species (hydrogen peroxide) produced during lipid peroxidation readily attacks the polyunsaturated fatty acids within the phospholipid bilayer causing the commencement of a self-propagating chain reaction within the membrane due to CH radicals reacting with O2 radicals producing peroxyl radicals (AW, 1998). Due to the self-propagating nature of the reaction series small lipid peroxidation can cause serious tissue damage resulting in atherosclerosis, asthma or kidney disease. Antioxidant activity quenches molecular oxygen (Yamauchi, 2010), and helps in the stabilisation of lipid-peroxyl free radicals via inhibition. Quercetin, a plant-derived aglycone flavonoid (Zhang M, 2011) was compared to aTocopherol (vitamin E) in the lipid peroxidation of rat liver homogenate. The liver metabolises materials and thus results in the production of free radicals when the oxidative balance is lost it leads to oxidative stress and thus having antioxidants to restore homoeostasis is required. Antioxidants have a high affinity for free radicals (Muriel, 2015) due to their ability to donate electrons. The antioxidant a-Tocopherol reduces oxidation under strong oxidative conditions, reducing the number of free radicals to be free at the end of lipid peroxidation. The data in figure 2 shows the average OD including Stdev bars, the variation in tubes 4-5 indicates poor experimental practice resulting in poor repeats within the data set and thus increasing variation within the data set. It suggests high oxidative conditions in tube 3 producing high concentrations of MDA (nM/ml) as seen in figure 4. Figure 4 also evidences that in the presence of a-Tocopherol lipid peroxidation is reduced as a reduction of MDA (the final product of lipid peroxidation and would result in pink adduct) is being produced suggest an interruption in the self-probating cycle of the fatty acids within the liver homogenate. This reduction is evidence as MDA concentration goes from a peak of 45nM/ml to an MDA reduction 24nM/ml in the presence of a-Tocopherol. When comparing the two sets of Data SEM and SD is used in order to give a relative comparison between the two different groups due to them being undertaken under different conditions. Comparing figure 2 and figure 5 (which used SD) the variation in data set A was much more significant as the higher SD values indicating a large variation within the repeats evidencing low reliability. Figure 5s SD bars a smaller then figure 2 indicating less variation and an increased reliability of the obtain results. Though both sets of data (A-B) show that the highest OD was found to be within tube 3 indicating that Fe2+ and H2O2 produce the highest concentration of MDA (nM/ml). SEM of the two data sets show that the accuracy of the two groups are similar and both show a decline in MDA concentration in the presence of the antioxidant, evidencing a reduction in lipid peroxidation (MDA is the product of lipid peroxides metabolisation which results in the pink adduct) and free radical production in the presence of the chosen antioxidants. Using the calibration curve to gain the MDA concentration of each antioxidant shows that quercetin resulted in a total reduction of free radicals as the MDA concentration was reduced to that of the control (buffer solution). Comparing this to a-Tocopherol there was a reduction of nearly half free radical concentration. These results indicate that the levels of oxidative stress are reduced in the presence of antioxidants. Improvements that can be made include, not losing the individual samples which would have been used for comparison, increasing the amount of antioxidants used to show and overall reduction in free radicals in different antioxidants. Also individual human error resulted in data sets begin provided requiring more lab expertise would reduce this and thus reduce was and cost of the experiment. Antioxidants reduce the concentration of MDA (nM/ml) present in the test tube via the inhibition of oxidative stress and lipid peroxidation of the cell membrane lipids. Quercetin completely reduced local MDA concentration of the rat homogenate indication no lipid peroxidation was occurring due to the binding of antioxidant to the local free radicals (produced via the Fenton reaction) due to their naturally high affinity. There was also a noticeable reduction of MDA concentration in the presence of aTocopherol though this was only an estimated 50% reduction. It can be seen that antioxidants offer a level of cell lipid protection against free radicals and a reduction in oxidative stress, resulting in less overall tissue damage. References Antonio Ayala, M. F. (2014). Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal. Oxidative Medicine and Cellular Longevity, 2014(2014), 31. doi:http://dx.doi.org/10.1155/2014/360438 AW, G. (1998). Lipid hydroperoxide generation, turnover, and effector action in biological systems. The Journal of Lipid Research, 39(8), 1529-1542. Esterbauer H, G. J. (1992). The role of lipid peroxidation and antioxidants in oxidative modification of LDL. Free Radical Biology and Medicine, 13(4), 341-390. Justino GC, S. M. (2004). Plasma quercetin metabolites: structure-antioxidant activity relationships. Archives of Biochemistry and BIophysics `, 432(1), 109-121. doi:10.1016/j.abb.2004.09.007 Kwiecien S, J. K. (2014). Lipid peroxidation, reactive oxygen species and antioxidative factors in the pathogenesis of gastric mucosal lesions and mechanism of protection against oxidative stress induced gastric injury. Journal of Physiology and Pharmacology, 65(5), 613-622. Muriel, S. C.-G. (2015). Antioxidants in liver health. The World Journal of Gastrointestinal Pharmacology and Therapeutics, 6(3), 59-72. doi:10.4292/wjgpt.v6.i3.59 Mylonas C, K. D. (1999). Lipid peroxidation and tissue damage. In Vivo, 13(3), 295-309. Yamauchi, R. (2010). Functions of Antioxidant Vitamins against Lipid Peroxidation. (F. o. Science, Ed.) Foods Food Ingredients Japan, 215(1), 501-1193. Yngo J. Garciaa, A. J.-M. (2005). Lipid peroxidation measurement by thiobarbituric acid assay in rat cerebellar slices. Journal of Neuroscience Methods, 144(1), 127-135. Zhang M, S. S. (2011). Antioxidant properties of quercetin. Advances in Experimental Medicine and Biology, 701, 283-289. doi:doi: 10.1007/978-1-4419-7756-4_38.

Friday, September 20, 2019

Hadith Is What Prophet Muhammad Said Religion Essay

Hadith Is What Prophet Muhammad Said Religion Essay Chapter 3 Hadith is what Prophet Muhammad Sallallahu alaihi wa Sallam said. Sunnah is what Prophet Muhammad Sallallahu alaihi wa Sallam said, did, and approved of. When we read Hadith we learn about his sunnah. We want to learn about his Hadith because we want to follow what he did and what he approved of, in detail. This is all related to Islam and its about religion people from all the world wants to learn and to know more about Islam, hadith what is going to help learning the ethics of Islam, hadith has mentioned in Quran its referring to wisdom and who ever bring to us the hadith such as Muslim and bukhari are sure hundred percent this is what came in Quran about hadith: For Allah has sent down to you the Book and the Wisdom and taught you what you knew not (before): and great is the Grace of Allah upon you. Moreover God asked us to obtain him and to fallow what prophet mohammed has told us or to do what he has did in specific stations and those are some examples: You have indeed in the Messenger of Allah a beautiful example (of conduct) for anyone whose hope is in Allah and the final Day and remembers Allah much. Say: If you do love Allah, then follow me: Allah will love you and forgive you your sins, and Allah is Forgiving Merciful. O people! The Messenger has come to you with the truth from your Lord. Therefore believe, (it is) better for you. And if you disbelieve, still, lo! to Allah belongs whatsoever is in the heavens and the earth. And Allah is ever Knower, Wise. O you who believe! Obey Allah, and obey the Messenger and those of you who are in authority, then if you have a dispute concerning any matter, refer it to Allah and the Messenger if you are (in truth) believers in Allah and the Last Day. That is better and very good in the end. Whoso obeys the Messenger, indeed obeys Allah and whoso turns away: we have not sent you as a warder over them. O you who believe! Obey Allah and His Messenger, and turn not away from him when you hear ( him speak). O you who believe! Be not unfaithful to Allah and the Messenger, nor knowingly betray your trusts. O you who believe! Obey Allah and obey the Messenger and render not your deeds useless. And whatsoever the Messenger gives you, take it. And whatsoever he forbids, abstain (from it). And fear Allah. Lo! Allah is severe in retributing (evil) For this reason the Quran is the first source of Muslim law and Hadith is the second source of Muslim law. Whatever Prophet Muhammad Sallallahu alaihi wa Sallam said, did or approved of was narrated by his family members and companions as Hadith. The man who narrated the most number of Hadith of Prophet Muhammad Sallallahu alaihi wa Sallam was Hadrat Abu Huraira Rady Allahu Anhu, and the lady who narrated the most Hadith of Prophet Muhammad Sallallahu alaihi wa Sallam was Sayyidatina Aisha Rady Allahu Anha. (web site 9) 3.2 Books There are six major books of Hadith. These books are: Sahih Bukhari by Imam Bukhari (194 A.H.-256 A.H.) containing 7275 Hadith, Sahih Muslim by Imam Muslim (206 A.H.-261 A.H.) containing 4000 Hadith, Jami by Imam Tirmizi (209 A.H-279 A.H.) containing 1600 Hadith, Sunan by Imam Abu Dawud (202 A.H.-275 A.H.) containing 4800 Hadith, Sunan by Imam Ibnu Maja (passed away 283 A.H.), and finally Sunan by Imam An-Nasaai (215 A.H.-303 A.H.). Some of the things that you learn from Hadith are that to say that God is one and prophet mohammed is who brought the Islam and that called shhada and is the key to Paradise, how to say our prayer, what is zakah which means give the poor people a percentage from our money every year and how much to give in zakah, the importance of fasting in the month of Ramadan, and how to perform Hajj. We also learn about Arkanal Iman or the pillars of faith, and how to be good Muslims by practicing Ihsan (goodness). We learn about the things that are prohibited in Islam such as stealing, cheating, lying, back-biting, fraud, alcohol and pork. The other thing that we learn from Hadith is what to do or recite on specific occasions for example: when giving in charity, say Fisabilillah. Those are the most trusted book that we can refer to when we want to learn hadith or when we want to know what is the low that we can follow in our lives and how to deal or treat people. Finally, Prophet Muhammad Sallallahu alaihi wa Sallam believed that Allah is dearer to him than everything on which the sun rises. (Muslim) Prophet Muhammad Sallallahu alaihi wa Sallam used to say, Glory to be to Allah; Praise be to Allah; there is no one worthy of worship but Allah; and Allah in most Great, is dearer to me than everything on which the sun rises. (Muslim) Prophet Muhammad Sallallahu alaihi wa Sallam said if anyone says this 100 times a day, his sins will be removed from him. (Muslim) Prophet Muhammad Sallallahu alaihi wa Sallam taught us that there is no might and no power other than Allah. Hadith is a very important aspect of being a Muslim because it develops love for Allah Subhanahu wa Taala and for Prophet Muhammad Sallallahu alaihi wa Sallam. Following the sayings of Prophet Muhammad Sallallahu alaihi wa Sallam makes you follow the right path.(web site 10) 3.3. Alrwah Alrwah are the people to bring to us the hadith from years back where prophet mohammed where living alrwah is the most honest people, this processes done by one of the rwah told that prophet mohammed told someone what he is tilling right now and the final six rwah who wrote what they know in a books that will last forever and be used all over the world (website 11), and those rwah are listed below: Muslim Bukhari Imam tirmzi Imam abu dawood Ibn majed An-Nasaai 3.4. Our module Ontological modeling in information technologies has undergone considerable evolution. Models and languages used for ontology construction and reasoning can be classified. Our module will be about prophet mohammed says and the rwah by which we get the specific hadith and this are the components that we used in our model. 3.5. Our Ontology Components Regardless of where and when an ontology is used or created, most share the same structural similarities, regardless of the language or object. Common components include: Individuals Instances, or the basic ground level component of an ontology. It may include concrete objects, such as animals, people, tables, automobiles, etc, as well as abstract individuals such as numbers and words. Classes Concepts that are also called type, sort, category and kind that is defined as an extension abstract groups, sets, or collection objects or intension abstract objects that are defines by values of aspects. Some examples of classes may include: Person: class of all people or abstract object that can be described by the criteria of being a person. Vehicle: class of all vehicles or the abstract object that can described by the criteria for being a vehicle Attributes Objects in an ontology can be described by relating them to other things, typically aspects or parts. These related things are often called attributes, although they may be independent. Each attribute can be either a class or an individual and will determined the king of relation between them. Without attributes, they will not be considered as a true ontology. Relationships A relation is of a particular type or class that specifies in what sense the object if related to another object in the ontology. The set of relations in an ontology will describe the semantics of the domain. The classes of relations used will describe the language in which the ontology is expressed. Function terms Complex structures formed from certain relations that can be used in place of an individuals term in a statement Restrictions Formally stated descriptions of what must be true for us to know what should be accepted as input. Rules Statements in the form of if-then sentences to describe the logical interferences in an ontology. Events The changing of attributes or relations. we can classified our model to be Structural (object) models Several approaches are known to apply structural (object) data models to define ontologies. An approach for extensible ontological model construction in a mediation environment intended for heterogeneous information sources integration in various subject domains has been presented in [kalin]. A mediator ontological language (MOL) may depend on a subject domain and is to be defined at the mediator consolidation phase. On the other hand, for different information sources different ontological models (languages) can be used to define their own ontologies The proposed model extends the usual ontology models and has been implemented in a frame-based knowledge model inspired to OKBC. The extension concerns the explicit representation of additional information about the properties of the attributes used to describe a concept. The ontology model extension encompasses semantic information designed to characterise the behavior of properties in agents concept descriptions. The novelty of this extended knowledge model is that it explicitly represents the behaviour of attributes over time by describing the changes in a property that are permitted for members of the concept. It also explicitly represents the class membership mechanism by associating with each slot a qualitative quantifier representing how properties are inherited by subconcepts. Finally, the model does not only describe the prototypical properties holding for a concept but also the exceptional ones. (ref 5,6) 3.6. graph explanation (fig 2) root of the ontology for an explanation to this graph which is our model a thing is the beginning of any ontology, on our ontology a thing will refer to an Islamic famous people and alsnah alnboia which is contain prophet mohammed says, Islamic famous people will be alsahabh (prophet mohammed fiends), alrwah and prophet Mohamed himself. (fig 3) prophet mohammed class This will take us to prophet mohammed says which will contain his life for an example and anything referring van be added to modify the ontology in this can be done by any one. (Fig 4) branches of our ontology Moreover from the famous Islamic people the rwah will show the rwah name and we can add any thing related to alrwah under the name, and the names of alshaba will be the same and later we will explain the relation and how we will connect each class to others. Finally, for alsnh the says will come under this class and the says will be divided to hadith books name and all hadith related to this books, and those will be linked with what we talked about such as alrwah, alshabh, prophet mohammed etc.

Thursday, September 19, 2019

Life in the 1960s was Better than Life Today Essay examples -- essays

Life in the 1960s was both better for the people and significantly different to life today. When comparing the 1960s and today, there are many significant differences. The 1960s held events that were unique to that era, such as the Vietnam War and the landing on the moon, and today we are trying to find ways to advance technology further. The two eras also had different lifestyles, clothing, technology and pass-times. Life in the 1960s was better than the life today because the world hadn?t yet advanced too far, and life was revolved more around friends and family then other things. Events in the 1960s changed the people of that era. People who did adventurous things such as Neil Armstrong walking on the moon and those men who returned home from the Vietnam War inspired them, feminism bettered the life for women, teens began to enjoy life more as the counter culture began, the cold war made people aware of the danger of nuclear technology and the JFK assassination gave people a topic of conversation. Important events today include the discovery of cloning, which is a way of c...

Wednesday, September 18, 2019

Reactions to Death in Faulkner’s As I Lay Dying :: Faulkner’s As I Lay Dying

Reactions to Death in Faulkner’s As I Lay Dying Eventhough As I lay Dying is a story revolved around the death of a mother, Addie Bundren, the true content of the story develops from information given to the audience through a multitude of narrators. The relationship between each family member and their association with Addie's death differentiates from person to person. Through her husband, Anse, we are introduced to his philosophy that man should keep stationary. Anse explains, "The lord put roads for travelling; why he laid them down flat on the Earth. When he aims for something to be always a-moving, he makes it long ways, like a road or a horse or a wagon, but when he aims for something to stay put, He makes it up and down ways, like a tree or a man" (24). In comparing a tree to a man, Anse feels that eventhough alive, man was not created to move about. This idea exhibits a lack of suspense within Anse, which may or may not have been Annie's desire to have an affair which lead to the birth of her child Jewel. Interestingly, A nse contradicts his own beliefs to fullfill his wife's wishes to be layed to rest next to her own family. This act can also be seen as selfish when Anse really desires a new set of teeth. Other narrators's ideas on life are presented through the death of Annie. For example, DArl represents seperation from the rest of the world. Riding with Jewel, DArl suggests that, "It takes two people to make you, and one people to die. That's how the world is going to end" (27). In this thought, Darl explains that even though a person is created by the life of two people, in the end, that life will die on its own. The fact that Darl is so open to talk about his surety of Annie's approaching death, distinguishes him from Jewel or Dewy Dell, who cannot speak about it. Darl therefore is an outcast of the family, which is later explained by his participation in the war. By writing As I Lay Dying through the thoughts of a variety of narrators, William

Tuesday, September 17, 2019

Hplc

Analysis   and   Separation   of   Organic   Acids   in   White   Wine   Using   High  Performance  Liquid  Chromatography Atis,  Arnelson  Arwin  G. and  Gaitos,  Gerald  M. Institute  of  Chemistry,  University  of  the  Philippines,  Diliman,  Quezon  City   Performed:  December  9,  2011   Submitted:  December  15,  2011 RESULTS  AND  DISCUSSION   solutions   containing   increasing   amounts   of   the   organic   acid. These   solutions   were   contained   in   flasks   numbered   5,   6,   7,   8,   and   9. (Refer   to   the   Appendix   to   see   components   of   each   flask). Tables   2,   3,   4,   and   5   show  the  peak  areas  and  the  equation  of  the  calibration   curve   obtained   for   each   organic   acid   in   the   chromatograms  obtained  for  flasks  5  to  9. Table  2. Peak  Areas  of  Tartaric  Acid  in  Flasks  5,  6,  7,  8,   and  9   Flask   Retention   Peak  Area   Concentration   time  (min)   (g/L)   5   2. 863   491  364   0. 5   6   2. 905   918  187   1   7   3. 027   5  949  164   5   8   3. 044   8  363  261   8   9   3. 065   9  475  446   10   Calibration  Curve  Equation:  Ã‚   y  =  984  371. 3561x  +  216  064. 755   R2  =  0. 9912      Table  3. Peak  Areas  of  Malic  Acid  in  Flasks  5,  6,  7,  and   8   Flask   Retention   Peak  Area   Concentration   time  (min)   (g/L)   5   3. 02    159742   0. 5   6   3. 760   1  114  051   1   7   3. 756   4  892  727   5   8   3. 795   6  883  424   10   Calibration  Curve  Equation:  Ã‚   y  =  688  260. 8175x  +  475  029. 6892   R2  =  0. 9766      Table   4. Peak   Areas   of   Lactic   Acid   in   Flasks   5,   6,   7,   8,   and  9   Flask   Retention   Peak  Area   Concentration   time  (min)   (g/L)   5   3. 533   575  620   0. 1   6   3. 598   1  248  888   1   7   3. 621   3  762  922   2. 5 The   retention   times   of   different   organic   acids   commonly   found   in   white   wine   were   obtained   using   high   performance   liquid   chromatography. The   peak   areas   of   the   acids   were   then   obtained   through   chromatograms  and  plotted  against  the  concentrations   of   the standard   solutions   of   the   organic   acids   to   construct   a   calibration   curve. Finally,   the   calibration   curves   obtained   were   used   to   determine   the   concentration   of   the   organic   acids   in   samples   of   white   wine. Standard   solutions   of   various   organic   acids   commonly   found   in   white   wine   (tartaric,   malic,   lactic,   citric)   were   assigned   to   be   contained   in   flasks   1,   2,   3,   and   4,   respectively,   and   were   run   through   the   chromatograph. The  mobile  phase  used  for  the  analytes   was   H3PO4   at   pH   3,   to   prevent   dissociation   of   the   organic   acids. The   resulting   chromatograms   of   each   standard   were   then   analyzed   to   obtain   the   retention   times   of   the   organic   acids. Table   1   shows   the   experimental   retention   times   of   the   different   organic   acids. Table   1. Experimental   Retention   Times   of   Organic   Acids   Organic  Acid   Retention  Time  (min)   Tartaric  Ã‚   3. 088   Malic   3. 812   Lactic   3. 620   Citric   3. 68      Since   there   were   no   clear   peaks   from   the   chromatograms   obtained   for   the   tartaric,   malic,   and   lactic   acid   samples,   the   retention   time   at   which   the   peak   height   is   greatest   was   obtained   as   the   experimental  retention  time  of  the  organic  acids. The   experimental   retention   times   were   then   used   to   identify   the   peak   areas   corresponding   to   each   organic  acid  analyzed  in  the  resulting  chromatograms  of 8   3. 597   5  048  614   3   9   3. 619   6  519  896   5   Calibration  Curve  Equation:  Ã‚   y  =  1  300  341. 246x  +  414  396. 3089   R2  =  0. 9815      Table   5. Peak   Areas   of   Citric   Acid   in   Flasks   5,   6,   7,   8,   and  9   Flask   Retention   Peak  Area   Concentration   time  (min)   (g/L)   5   3. 140   361  921   0. 05   6   3. 208   907  232   0. 1   7   3. 306   4  736  427   0. 5   8   3. 325   7  508  481   0. 75   9   3. 347   9  612  454   1   Calibration  Curve  Equation:  Ã‚   y  =  9  836  731. 501x  +  96  328. 12036   R2  =  0. 9994      The   equations   of   the   calibration   curves   were   then   used   to   calculate   the   concentration   of   each   organic   acid   in   the   white   wine   samples. The   peak   area   corresponding   to   each   organic   acid   was   first   obtained,   and   substituted   in   the   calibration   curve   equation. The   concentration  of  each  acid  is  shown  in  Table  6. Table   6. Experimental   Concentration   of   Various Organic  Acids  in  White  Wine  Samples. Organic   Retention   Peak  Area   Concentration   Acid   Time  (min)   (g/L)   Tartaric  Ã‚   2. 966   2  675  651   2. 499   Malic   3. 752   3  576  021   4. 506   Lactic   3. 393   4  709  737   3. 303   Citric   3. 144   794  394   0. 091      The  results  indicate  that  malic  acid  is  the  major   component  of  white  wine. But  in  reality,  this  is  not  the   case. The  major  component  of  white  wine  is  found  to  be   tartaric  acid. The   chromatograms   (See   Appendix)   of   flasks   1   to   9,   and   of   the   sample   are   not   well   resolved. This   discrepancy   may   be   caused   by   several   factors. These   factors   include   poor   solution   preparation,   contamination  of  the  solvent  or  the  sample,  bubbles  in   the   detector,   impurities   in   the   mobile   phase,   bleeding   of  the  column,  inadequate  adjustment  of  equilibrium  in   gradient   operation,   and   carry? over   from   previous   injection. Due  to  these  factors,  it  is  highly  advised  that  the   future   researchers   should   cautiously   execute   each procedure   of   the   experiment   to   eliminate   the   discrepancy   and   accordingly,   they   could   attain   better   results. They   could   also   make   use   of   theoretical   retention  times  of  the  organic  acids  to  determine  each   of   them   and   which   could   further   help   the   future   researchers   to   analyze   the   wine   sample   more   efficiently. REFERENCE:      Meyer,  Veronika  R. Practical  High  Performance  Liquid  Ã‚  Ã‚     Ã‚   Chromatography. 2nd  ed. 1993. England:  John  Ã‚   Wiley  &  Sons  Ltd. APPENDIX  Ã‚   Data  Sheets  Ã‚      Concentration  of  tartaric  acid  standard:  50. 0  g/L   Concentration  of  malic  acid  standard:  50. 0  g/L   Concentration  of  lactic  acid  standard:  25. 0  g/L   Concentration  of  citric  acid  standard:  5. 0  g/L   Composition  of  Flasks  1? 9   Volume  of  standard  stock  solutions  (in  mL)      Retention  Time  Measurement   Calibrat ion  Curve   Flask  #   1   2   3   4   5   6   7   8   Tartaric  Acid   5. 00   0. 00   0. 00   0. 00   0. 25 0. 50 2. 50 4. 00 Malic  Acid   0. 00   7. 50   0. 00   0. 00   0. 10 0. 50 2. 50 5. 00 Lactic  Acid   0. 00   0. 00   5. 00   0. 00   0. 10 1. 00 2. 50 3. 00 Citric  Acid   0. 00   0. 00   0. 00   5. 00   0. 25 0. 50 2. 50 3. 75 9   5. 00 7. 50 5. 00 5. 00 *Chromatograms  of  flasks  1? 9  and  of  the  white  wine  sample  can  be  seen  in  the  remaining  pages  after  this.